Affiliation:
1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 75 Xiuling Road, Nanning 530005, China
2. Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi University, 75 Xiuling Road, Nanning 530005, China
Abstract
Oxidative stress degrades oocytes during in vitro maturation (IVM). Catalpol, a well-known iridoid glycoside, exhibits antioxidant, anti-inflammatory, and antihyperglycemic effects. In this study, catalpol supplementation was tested on porcine oocyte IVM and its mechanisms. Corticalgranule (GC) distribution, mitochondrial function, antioxidant capacity, DNA damage degree, and real-time quantitative polymerase chain reaction were used to confirm the effects of 10 μmol/L catalpol in the maturation medium during IVM. Catalpol treatment significantly increased the first-pole rate and cytoplasmic maturation in mature oocytes. It also increased oocyte glutathione (GSH), mitochondrial membrane potential and blastocyst cell number. However, DNA damage as well as reactive oxygen species (ROS) and malondialdehyde (MDA) levels. Mitochondrial membrane potential and blastocyst cell number were also increased. Thus, the supplementation of 10 μmol/L catalpol in the IVM medium improves porcine oocyte maturation and embryonic development.
Funder
National Natural Science Foundation of China
Innovation Project of Guangxi Graduate Education
Subject
Cell Biology,Clinical Biochemistry,Molecular Biology,Biochemistry,Physiology
Reference55 articles.
1. Supplementation with Niacin during in vitro maturation improves the quality of porcine embryos;Almubarak;Theriogenology,2021
2. Review: Recent advances in bovine in vitro embryo production: Reproductive biotechnology history and methods;Kjelland;Anim. Int. J. Anim. Biosci.,2020
3. Utility of antioxidants during assisted reproductive techniques: An evidence based review;Agarwal;Reprod. Biol. Endocrinol. RBE,2014
4. Cao, B., Qin, J., Pan, B., Qazi, I.H., Ye, J., Fang, Y., and Zhou, G. (2022). Oxidative Stress and Oocyte Cryopreservation: Recent Advances in Mitigation Strategies Involving Antioxidants. Cells, 11.
5. Activation of apoptosis signalling pathways by reactive oxygen species;Biochim. Biophys. Acta,2016
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