Affiliation:
1. College of Life Science, Southwest Forestry University, Kunming 650224, China
Abstract
This study investigated the regulatory mechanism of myristic acid on milk fat synthesis in cows. An association between myristic acid and high milk fat content in Zhongdian yaks’ guts was found through combined metagenomic and metabolomic analysis. Bovine mammary epithelial cells (MAC-T) were cultured and treated with various myristic acid concentrations. After 24 h, the protein expression levels of CD36 (membrane glycoprotein CD36), ADFP (adipose differentiation-related protein), and UB (ubiquitin) were analyzed, along with cellular proteasome activity, triglyceride content, lipid droplets, and cell viability. Myristic acid at 200 μM significantly upregulated CD36, ADFP, UB, the content of triglyceride content and lipid droplets, and cell viability, but did not affect proteasome activity. Pathway analysis revealed that myristic acid regulates milk fat synthesis through ubiquitination–lysosome and ubiquitination–proteasome pathways. The study demonstrates myristic acid’s role in regulating triglyceride synthesis in MAC-T cells and its potential application as a feed additive for cattle, benefitting the dairy industry’s milk production efficiency and economic outcomes.
Funder
National Natural Science Foundation
Scientific Research Foundation of Southwest Forestry University
Ten Thousand Talent Plans for Young Top-notch Talents of Yunnan Province
Anhui Natural Science Foundation
Subject
Plant Science,Agronomy and Crop Science,Food Science
Cited by
1 articles.
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