Affiliation:
1. Department of Earth and Environmental Sciences (DSTA), University of Pavia, Via A. Ferrata 9, 27100 Pavia, Italy
2. Laboratory of Food Chemistry, Department of Chemistry and Technology of Drugs, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
3. NMR-Based Metabolomics Laboratory (NMLab), Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy
4. MOGU S.r.l., Via S. Francesco d’Assisi 4, 21020 Inarzo, Italy
5. Department of Biology and Biotechnology “L. Spallanzani”, University of Pavia, 27100 Pavia, Italy
Abstract
Wood Decay Fungi (WDF) are fungi specialized in degrading wood. An interesting perspective is their use as a source of Novel Foods or food ingredients. Here, for the first time, the metabolite profiling of hydroalcoholic and organic extracts from A. biennis, F. iberica, S. hirsutum mycelia was investigated by NMR methodology. Amino acids (alanine, arginine, asparagine, aspartate, betaine, GABA, glutamate, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, tyrosine, valine), sugars (galactose, glucose, maltose, trehalose, mannitol), organic acids (acetate, citrate, formate, fumarate, lactate, malate, succinate), adenosine, choline, uracil and uridine were identified and quantified in the hydroalcoholic extracts, whereas the 1H spectra of organic extracts showed the presence of saturated, mono-unsaturated and di-unsaturated fatty chains, ergosterol,1,2-diacyl-sn-glycero-3-phosphatidylethanolamine, and 1,2-diacyl-sasglycero-3-phosphatidylcholine. A. biennis extracts showed the highest amino acid concentration. Some compounds were detected only in specific species: betaine and mannitol in S. hirsutum, maltose in A. biennis, galactose in F. iberica, GABA in F. iberica and S. hirsutum, and acetate in A. biennis and S. hirsutum. S. hirsutum showed the highest saturated fatty chain concentration, whereas DUFA reached the highest concentration in A. biennis. A high amount of ergosterol was measured both in A. biennis and F. iberica. The reported results can be useful in the development of WDF-based products with a high nutritional and nutraceutical value.
Funder
Fondazione Cariplo and Regione Lombardia
MYCO-ADVANCED LEATHER MATERIALS
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference36 articles.
1. Wood decay fungi: An analysis of worldwide research;Li;J. Soils Sediments,2022
2. Characterization of mycelia from wood-decay species by TGA and IR spectroscopy;Girometta;Cellulose,2020
3. EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) (2010). Scientific Opinion on the safety of ‘Chitin-glucan’ as a Novel Food ingredient. EFSA J., 8, 1687.
4. Safety of vitamin D2 mushroom powder as a novel food pursuant to Regulation (EU) 2015/2283;Turck;EFSA J.,2020
5. (2023, April 26). European Commision Consultation Process on Novel Food Status. Available online: https://food.ec.europa.eu/safety/novel-food/consultation-process-novel-food-status_en.