Characterization of Physicochemical, Biological, and Chemical Changes Associated with Coconut Milk Fermentation and Correlation Revealed by 1H NMR-Based Metabolomics
Author:
Qadi Wasim1, Mediani Ahmed2ORCID, Benchoula Khaled3, Wong Eng3, Misnan Norazlan4ORCID, Sani Norrakiah1
Affiliation:
1. Department of Food Science, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi 43650, Malaysia 2. Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, UKM Bangi, Bangi 43600, Malaysia 3. School of Medicine, Faculty of Health and Medical Sciences, Taylor’s University, 1, Jalan Taylors, Subang Jaya 47500, Malaysia 4. Herbal Medicine Research Centre, Institute for Medical Research, National Institutes of Health, Shah Alam 40170, Malaysia
Abstract
Fermentation of milk enhances its nutritional and biological activity through the improvement of the bioavailability of nutrients and the production of bioactive compounds. Coconut milk was fermented with Lactiplantibacillus plantarum ngue16. The aim of this study was to evaluate the effect of fermentation and cold storage for 28 days on physicochemical characteristics, shelf life, and antioxidant and antibacterial activities of coconut milk as well as its proximate and chemical compositions. The pH of fermented milk decreased from 4.26 to 3.92 on the 28th day during cold storage. The viable cell count of lactic acid bacteria (LAB) in fermented coconut milk was significantly increased during fermentation and cold storage period (1 to 14 days), reaching 6.4 × 108 CFU/mL, and then decreased significantly after 14 days to 1.6 × 108 CFU/mL at 28 days. Yeast and molds in fermented coconut milk were only detected on the 21st and 28th days of cold storage, which ranged from 1.7 × 102 to 1.2 × 104 CFU/mL, respectively. However, the growth of coliforms and E. coli was observed on the 14th until the 28th day of cold storage. The fermented coconut milk demonstrated strong antibacterial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Cronobacter sakazakii, Bacillus cereus, and Salmonella typhimurium compared to fresh coconut milk. Fermented coconut milk had the greatest 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) values, with 67.1% and 61.961 mmol/g at day 14 of cold storage, respectively. Forty metabolites were detected in fermented and pasteurized coconut milk by proton nuclear magnetic resonance (1H NMR) metabolomics. The principal component analysis (PCA) showed clear difference between the fermented and pasteurized coconut milk as well as the studied cold storage days. The metabolites responsible for this variation were ethanol, valine, GABA, arginine, lactic acid, acetoin, alanine, phenylalanine, acetic acid, methionine, acetone, pyruvate, succinic acid, malic acid, tryptophan, uridine, uracil, and cytosin, which were higher in fermented coconut milk. However, sugars and other identified compounds were higher in fresh coconut milk. The findings of this study show that fermentation of coconut milk with L. plantarum ngue16 had high potential benefits to extending its shelf life and improved biological activities as well as other beneficial nutrients.
Funder
Ministry of Higher Education (MoHE), Malaysia
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science
Reference69 articles.
1. Bibra, M., Krishnaraj, R.N., and Sani, R.K. (2021). Biomolecular Engineering Solutions for Renewable Specialty Chemicals: Microorganisms, Products, and Processes, John Wiley & Sons, Inc. 2. How well do plant based alternatives fare nutritionally compared to cow’s milk?;Vanga;J. Food Sci. Technol.,2018 3. Bartkiene, E., Lele, V., Ruzauskas, M., Domig, K.J., Starkute, V., Zavistanaviciute, P., Bartkevics, V., Pugajeva, I., Klupsaite, D., and Juodeikiene, G. (2019). Lactic acid bacteria isolation from spontaneous sourdough and their characterization including antimi-crobial and antifungal properties evaluation. Microorganisms, 8. 4. Review on lactic acid bacteria function in milk fermentation and preservation;Teshome;Afr. J. Food Sci.,2015 5. Production of coconut milk: A sustainable alternative plant-based milk;Tulashie;Case Stud. Chem. Environ. Eng.,2022
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