Natural Sunlight-Mediated Emodin Photoinactivation of Aeromonas hydrophila

Author:

Urgesa Gelana12ORCID,Lu Liushen2,Gao Jinwei12ORCID,Guo Lichun3,Qin Ting2,Liu Bo2ORCID,Xie Jun2,Xi Bingwen12ORCID

Affiliation:

1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China

2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

3. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China

Abstract

Aeromonas hydrophila can be a substantial concern, as it causes various diseases in aquaculture. An effective and green method for inhibiting A. hydrophila is urgently required. Emodin, a naturally occurring anthraquinone compound, was exploited as a photo-antimicrobial agent against A. hydrophila. At the minimum inhibitory concentration of emodin (256 mg/L) to inactivate A. hydrophilia in 30 min, an 11.32% survival rate was observed under 45 W white compact fluorescent light irradiation. In addition, the antibacterial activity under natural sunlight (0.78%) indicated its potential for practical application. Morphological observations demonstrated that the cell walls and membranes of A. hydrophila were susceptible to damage by emodin when exposed to light irradiation. More importantly, the photoinactivation of A. hydrophila was predominantly attributed to the hydroxyl radicals and superoxide radicals produced by emodin, according to the trapping experiment and electron spin resonance spectroscopy. Finally, a light-dependent reactive oxygen species punching mechanism of emodin to photoinactivate A. hydrophila was proposed. This study highlights the potential use of emodin in sunlight-mediated applications for bacterial control, thereby providing new possibilities for the use of Chinese herbal medicine in aquatic diseases prevention.

Funder

Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS

Liushen Lu

Postdoctoral Fellowship Program of CPSF

Lichun Guo

Publisher

MDPI AG

Reference51 articles.

1. Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review;Stratev;J. Infect. Public Health,2016

2. Multidrug-resistant Aeromonas hydrophila causing fatal bilateral necrotizing fasciitis in an immunocompromised patient: A case report;Perry;J. Med. Case Rep.,2018

3. Rasmussen-Ivey, C., Figueras, M., McGarey, D., and Liles, M. (2016). Virulence factors of Aeromonas hydrophila: In the wake of reclassification. Front. Microbiol., 7.

4. Mzula, A., Wambura, P., Mdegela, R., and Shirima, G. (2019). Current state of modern biotechnological-based Aeromonas hydrophila vaccines for aquaculture: A systematic review. BioMed Res. Int., 2019.

5. Isolation, biochemical and molecular detection of Aeromonas hydrophila from cultured Oncorhynchus mykiss;Zorriehzahra;Iran. J. Fish. Sci.,2020

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3