Immunohistochemistry Reveals TRPC Channels in the Human Hearing Organ—A Novel CT-Guided Approach to the Cochlea

Author:

Englisch Colya N.1ORCID,Steinhäuser Jakob1,Wemmert Silke2ORCID,Jung Martin3ORCID,Gawlitza Joshua4,Wenzel Gentiana2,Schick Bernhard2,Tschernig Thomas1ORCID

Affiliation:

1. Institute of Anatomy and Cell Biology, Saarland University, 66421 Homburg/Saar, Germany

2. Department of Otorhinolaryngology, Head and Neck Surgery, Saarland University, 66421 Homburg/Saar, Germany

3. Institute of Medical Biochemistry and Molecular Biology, Saarland University, 66421 Homburg/Saar, Germany

4. Institute of Radiology, Technical University of Munich, 80333 Munich, Germany

Abstract

TRPC channels are critical players in cochlear hair cells and sensory neurons, as demonstrated in animal experiments. However, evidence for TRPC expression in the human cochlea is still lacking. This reflects the logistic and practical difficulties in obtaining human cochleae. The purpose of this study was to detect TRPC6, TRPC5 and TRPC3 in the human cochlea. Temporal bone pairs were excised from ten body donors, and the inner ear was first assessed based on computed tomography scans. Decalcification was then performed using 20% EDTA solutions. Immunohistochemistry with knockout-tested antibodies followed. The organ of Corti, the stria vascularis, the spiral lamina, spiral ganglion neurons and cochlear nerves were specifically stained. This unique report of TRPC channels in the human cochlea supports the hypothesis of the potentially critical role of TRPC channels in human cochlear health and disease which has been suggested in previous rodent experiments.

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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