Affiliation:
1. Department of Human Sciences, Ohio State University, Columbus, OH 43210, USA
2. Molecular and Cellular Imaging Center (MCIC), Ohio State University, Wooster, OH 44691, USA
Abstract
The intestinal microbial population is recognized for its impact on cancer treatment outcomes. Little research has reported microbiome changes during cancer progression or the interplay of disease progression, dietary sugar/fat intake, and the microbiome through surgery and chemotherapy. In this study, the murine gut microbiome was used as a model system, and changes in microbiome diversity, richness, and evenness over the progression of the cancer and treatment were analyzed. Mice were categorized into four diet cohorts, combinations of either high or low sucrose and high or low omega-3 fatty acids, and two treatment cohorts, saline vehicle or chemotherapy, for a total of eight groups. Fecal samples were collected at specific timepoints to assess changes due to diet implementation, onset of cancer, lumpectomy, and chemotherapy. Akkermansia muciniphila abundance was very high in some samples and negatively correlated with overall Amplicon Sequence Variant (ASV) richness (r(64) = −0.55, p = 3 × 10−8). Throughout the disease progression, ASV richness significantly decreased and was impacted by diet and treatment. Alpha-diversity and differential microbial abundance were significantly affected by disease progression, diet, treatment, and their interactions. These findings help establish a baseline for understanding how cancer progression, dietary macronutrients, and specific treatments impact the murine microbiome, which may influence outcomes.
Funder
National Institute of Health
Subject
Food Science,Nutrition and Dietetics
Reference70 articles.
1. Cancer Statistics, 2022. CA;Siegel;Cancer J. Clin.,2022
2. (2022, June 09). National Cancer Institute SEER Cancer Stat Facts: Female Breast Cancer, Available online: https://seer.cancer.gov/statfacts/html/breast.html.
3. The Occurrence and Prevention of Foodborne Disease in Vulnerable People;Lund;Foodborne Pathog. Dis.,2011
4. Qualitative Studies of the Food Safety Knowledge and Perceptions of Transplant Patients;Chen;J. Food Prot.,2010
5. Food Safety Issues for Cancer and Organ Transplant Patients;Medeiros;Nutr. Clin. Care,2004
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