Abstract
Sericin is a natural protein with high application potential, but the research on its efficacy is very limited. In this study, the anti-inflammatory mechanism of sericin protein was investigated. Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein. Finally, RNA-seq was performed to quantify the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages. The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways. The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment. SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines. It was confirmed that sericin inhibited LPS-induced inflammation through MyD88/NF-κB pathway. This finding provides necessary theoretical support for sericin development and application.
Funder
National Natural Science Foundation of China
Chongqing Research Program of Basic Research and Frontier Technology
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference54 articles.
1. Silk structure and degradation;Liu;Colloids Surf. B Biointerfaces,2015
2. Zhang, Y., Zhao, P., Dong, Z., Wang, D., Guo, P., Guo, X., Song, Q., Zhang, W., and Xia, Q. (2015). Comparative proteome analysis of multi-layer cocoon of the silkworm, Bombyx mori. PLoS ONE, 10.
3. The rheological properties of native sericin;Sparkes;Acta Biomater.,2018
4. Advances in silkworm studies accelerated by the genome sequencing of Bombyx mori;Xia;Annu. Rev. Entomol.,2014
5. Synergism of open chromatin regions involved in regulating genes in Bombyx mori;Zhang;Insect Biochem. Mol. Biol.,2019
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献