β-Hydroxy-β-Methylbutyric Acid Promotes Repair of Sheep Myoblast Injury by Inhibiting IL-17/NF-κB Signaling

Author:

Zheng JuanORCID,Li BoORCID,Yan Yiting,Huang Xiaoyu,Zhang Enping

Abstract

Delayed muscle development and impaired tissue repair are common occurrences in sheep reared for mutton. Therefore, understanding the regulatory mechanisms involved in muscle growth and development is critical for animal production. Skeletal muscle satellite cells (SMSCs) can simulate the proliferation and differentiation of muscle cells and could be induced to differentiate into myoblasts. β-hydroxy-β-methylbutyric acid (HMB) is an additive commonly used in animal production. This study examined the effect of HMB on myoblast injury repair using flow cytometry, EdU assay, RNA sequencing, Western blot, and ELISA. Our results showed that HMB could inhibit IL-17 expression and, in turn, inhibit NF-κB signaling. By acting on the downstream genes of NF-κB pathway IL-6, TNF-α and IL-1β, HMB inhibits the apoptosis and promotes the proliferation of myoblasts. The findings of this study provide insight into the mechanism by which HMB mediates myoblast injury repair in sheep.

Funder

Mutton Sheep Industrial Technology System Construction Project in Shaanxi Province

National Key Research and Development Program of China

Publisher

MDPI AG

Subject

Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis

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