Non-Affinity Purification of Antibodies-Reference-Cited by-同舟云学术

Non-Affinity Purification of Antibodies

Published:2023-02-13 Issue:1 Volume:12 Page:15
ISSN:2073-4468
Container-title:Antibodies
language:en
Short-container-title:Antibodies

Author:

Arakawa Tsutomu¹,Tomioka Yui²,Nakagawa Masataka²,Sakuma Chiaki²,Kurosawa Yasunori²,Ejima Daisuke³,Tsumoto Kouhei⁴,Akuta Teruo²

Affiliation:

1. Alliance Protein Laboratories, San Diego, CA 92130, USA

2. Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., Tahahagi 318-0004, Japan

3. Bio-Diagnostic Reagent Technology Center, Sysmex Corporation, Sayama 350-1332, Japan

4. Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo 113-8656, Japan

Abstract

Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-ethyl-pyridine (MEP) column chromatography.

Publisher

MDPI AG

Subject

Drug Discovery,Immunology,Immunology and Allergy

Link

https://www.mdpi.com/2073-4468/12/1/15/pdf

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