Isolation and Characterization of the Zearalenone-Degrading Strain, Bacillus spizizenii B73, Inspired by Esterase Activity
Author:
Liu Xue1, Wu Na2, Zhang Mingyu1, Xue Feng1, Xu Qing1
Affiliation:
1. School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210046, China 2. College of Marine and Bioengineering, Yancheng Institute of Technology, Yancheng 224007, China
Abstract
Zearalenone (ZEN) is a widespread mycotoxin found in grain and feed, presenting a serious threat to animal and human health. This study investigated the ability of the novel strain B73, isolated from petroleum-contaminated soil, to detoxify ZEN. B73 was identified as Bacillus spizizenii through physiological and biochemical tests, and further confirmed based on the 16S rRNA gene sequence and the complete genome sequence. B. spizizenii B73 was capable of degrading up to 99.3% of ZEN at a concentration of 10 μg/mL in a minimal medium (pH = 7.0) within 8 h at 37 °C via HPLC-UV. In addition, B. spizizenii B73 was used to treat ZEN-contaminated wheat bran, dried distillers grains (DDGS), and corn meal, whereby the respective degradation rates reached 96.32%, 98.73%, and 80.31% after 36 h of treatment. HPLC-Q-Exactive-MS/MS analysis revealed one of the degradation products to have the formula C17H24O4. B. spizizenii B73 is a novel strain isolated from petroleum-contaminated soil, and the extracellular enzymes secreted by this strain show a remarkable ability to degrade ZEN.
Funder
National Key Research and Development Program of China
Subject
Health, Toxicology and Mutagenesis,Toxicology
Reference52 articles.
1. Chang, H., Woori, K., Ju-Hee, P., Dongho, K., Choong-Ryeol, K., Soohyun, C., and Chan, L.J.T. (2017). The Occurrence of Zearalenone in South Korean Feedstuffs between 2009 and 2016. Toxins, 9. 2. Combined hazard assessment of mycotoxins and their modified forms applying relative potency factors: Zearalenone and T2/HT2 toxin;Steinkellner;Food Chem. Toxicol.,2019 3. Wang, M., Wu, W., Li, L., He, J., Huang, S., Chen, S., Chen, J., Long, M., Yang, S., and Li, P. (2019). Analysis of the miRNA Expression Profiles in the Zearalenone-Exposed TM3 Leydig Cell Line. Int. J. Mol. Sci., 20. 4. Occurrence and toxicity of a fusarium mycotoxin, zearalenone;Rai;Crit. Rev. Food Sci. Nutr.,2020 5. Mycotoxins and their effects on human and animal health;Rocha;Food Control,2014
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|