3D Printed Microfluidic Cell for SAXS Time-Resolved Measurements of the Structure of Protein Crystallization Solutions-Reference-Cited by-同舟云学术

3D Printed Microfluidic Cell for SAXS Time-Resolved Measurements of the Structure of Protein Crystallization Solutions

Published:2023-06-11 Issue:6 Volume:13 Page:938
ISSN:2073-4352
Container-title:Crystals
language:en
Short-container-title:Crystals

Author:

Marchenkova Margarita¹²,Chapek Sergei³^ORCID,Konarev Petr¹²^ORCID,Ilina Ksenia¹²,Peters Georgy²,Pisarevsky Yury¹²,Shishkov Vladimir¹,Soldatov Alexander³^ORCID,Kovalchuk Mikhail¹²

Affiliation:

1. A.V. Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics”, Russian Academy of Sciences, 59, Leninskii Prospect, 119333 Moscow, Russia

2. National Research Centre “Kurchatov Institute”, 1, Akademika Kurchatova pl., 123182 Moscow, Russia

3. The Smart Materials Research Institute, Southern Federal University, 178/124, Andreya Sladkova Street, 344090 Rostov on Don, Russia

Abstract

A multichannel microfluidic cell (MFC) obtained using 3D printing for studying the structure of complex solutions by small-angle X-ray scattering (SAXS) is described. MFC was tested at the BioMUR beamline of the Kurchatov synchrotron. A comparative analysis of SAXS signal from the standard capillary and from the developed MFC was carried out, with MFC showing significant advantages. The dynamics of SAXS scattering curves for lysozyme solutions with NaCl precipitant were studied when the protein and precipitant concentrations changed. The obtained time series of data are well consistent with the known data for the lysozyme solution.

Funder

Ministry of Science and Higher Education

Publisher

MDPI AG

Subject

Inorganic Chemistry,Condensed Matter Physics,General Materials Science,General Chemical Engineering

Link

https://www.mdpi.com/2073-4352/13/6/938/pdf

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