Abstract
This piece of research evaluates the presence of protease inhibitors in the macroalga Ulva ohnoi and provides an initial overview of their mode of action. The ability of Ulva protease inhibitors to inhibit digestive proteases of three marine fish species, as well as their capacity to hamper the hydrolysis of a reference protein by those fish proteases, were assessed. In addition, thermal stability and the mode of inhibition on trypsin and chymotrypsin were also studied. Dose-response inhibition curves and in vitro protein hydrolysis assays revealed a noticeable inhibition of fish enzymes when Ulva concentration increased in the assay. The thermal treatment of Ulva reduced markedly the inhibitory effect on fish digestive protease. Finally, Lineweaver–Burk plots indicated that trypsin and chymotrypsin inhibition consisted of a mixed-type inhibition mechanism in which the inhibitory effect depends on Ulva concentration. Overall, the results confirmed the presence of protease inhibitors in Ulva, though heat treatment was enough for inactivating these compounds.
Subject
Drug Discovery,Pharmacology, Toxicology and Pharmaceutics (miscellaneous),Pharmaceutical Science
Reference43 articles.
1. An overview of anti-nutritional factors in fish feed ingredients and their effects in fish;Prabu;J. Aquac. Trop.,2017
2. An overview of anti-nutritional factors in food;Thakur;Int. J. Chem. Stud.,2019
3. Risk assessment of the use of alternative animal and plant raw material resources in aquaculture feeds
4. A review on alternative plant protein sources available for future sustainable aqua feed production;Chakraborty;Int. J. Chem. Stud.,2019
5. Antinutritional factors in plant origin fish feed ingredients: The problems and probable remedies;Hajra;Adv. Fish Res.,2013
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献