3D Printed Device for Separation of Cells and Particles by Tilted Bulk Acoustic Wave Actuation

Abstract

Three-dimensional (3D) printing has been proven to be a reliable manufacturing method for a diverse set of applications in engineering. Simple benchtop tools such as mini centrifuges, automated syringe pumps, and basic-robotic platforms have been successfully printed by basic 3D printers. The field of lab-on-a-chip offers promising functions and convenience for point-of-care diagnostics and rapid disease screening for limited resource settings. In this work, stereolithography (SLA) 3D resin printing is implemented to fabricate a microfluidic device to be used for separation of HeLa cells from smaller polystyrene particles through titled angle standing bulk acoustic wave actuation. The demonstrated device achieved continuous and efficient separation of target cells with over 92% HeLa cell purity and 88% cell recovery rates. Overall, 3D printing is shown to be a viable method for fabrication of microfluidic devices for lab-on-a-chip applications.