Author:
Pu Lei,Luo Yunyan,Wen Zuochen,Dai Yuxin,Zheng Chunting,Zhu Xueli,Qin Lei,Zhang Chunguang,Liang Hong,Zhang Jianbin,Guo Liang,Wang Lixian
Abstract
GPX2 has been recognized as a potential candidate gene for feed efficiency in pigs. This article aimed to elucidate polymorphism of GPX2 associated with feed efficiency and its related molecular mechanism. In this study, seven single nucleotide polymorphisms (SNP) of GPX2 were found among 383 Duroc pigs. In addition, seven SNPs and ALGA0043483 (PorcineSNP60 BeadChip data in 600 Duroc pigs), which are near the GPX2 gene, were identified in one haplotypes block. Furthermore, associated studies showed that the genotype of GPX2 has significant association with weaning weight and 100 kg BF in Duroc pigs. In addition, the AG had no effect when the backfat became thinner, and the FCR and RFI traits had a tendency to decrease in the G3 + TT combination genotype, accompanied by an increase of GPX2 expression in backfat and muscle tissues. At the cellular level, the adipocyte proliferation and ability of adipogenic differentiation were reduced, and the lipid degradation increased in 3T3-L1 when there was overexpression of GPX2. In contrast, overexpression of the GPX2 gene can promote the muscle cell proliferation and myogenic differentiation in C2C12 cells. In other words, GPX2 has the effect of reducing fat deposition and promoting muscle development, and it is a candidate gene for backfat and feed efficiency.
Funder
Key R&D and Transformation Program of Qinghai Province Science and Technology Assistance Program for Qinghai, China
Tianjin Natural Science Foundation, China
Scientific Research Program of Tianjin Education Commission, China
Tianjin pig industry technology system innovation team, China
Subject
General Veterinary,Animal Science and Zoology
Cited by
3 articles.
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