A New Method for Growth Factor Enrichment from Dairy Products by Electrodialysis with Filtration Membranes: The Major Impact of Raw Product Pretreatment

Author:

Kadel Sabita12,Nichka Vladlen12ORCID,Thibodeau Jacinthe12,Parjikolaei Behnaz Razi3,Bazinet Laurent12ORCID

Affiliation:

1. Dairy Science and Technology Research Center (STELA), Institute of Nutrition and Functional Foods (INAF), Department of Food Sciences, Laval University, Quebec, QC G1V 0A6, Canada

2. Laboratory of Food Processing and ElectroMembrane Processes (LTAPEM), Laval University, Quebec, QC G1V 0A6, Canada

3. Arla Food Ingredients, Soenderupvej 26, DK-6920 Videbæk, Denmark

Abstract

This study is focused on fractionation of insulin-like growth factor I (IGF-I) and transforming growth factor-β2 (TGF-β2) using a new electro-based membrane process calledelectrodialysis with filtration membranes (EDFM). Before EDFM, different pretreatments were tested, and four pH conditions (4.25, 3.85, 3.45, and 3.05) were used during EDFM. It was demonstrated that a 1:1 dilution of defatted colostrum with deionized water to decrease mineral content followed by the preconcentration of GFs by UF is necessary and allow for these compounds to migrate to the recovery compartment during EDFM. MS analyses confirmed the migration, in low quantity, of only α-lactalbumin (α-la) and β-lactoglobulin (β-lg) from serocolostrum to the recovery compartment during EDFM. Consequently, the ratio of GFs to total protein in recovery compartment compared to that of feed serocolostrum solution was 60× higher at pH value 3.05, the optimal pH favoring the migration of IGF-I and TGF-β2. Finally, these optimal conditions were tested on acid whey to also demonstrate the feasibility of the proposed process on one of the main by-products of the cheese industry; the ratio of GFs to total protein was 2.7× higher in recovery compartment than in feed acid whey solution, and only α-la migrated. The technology of GF enrichment for different dairy solutions by combining ultrafiltration and electrodialysis technologies was proposed for the first time.

Funder

Arla Foods Ingredients

Publisher

MDPI AG

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