Basophil Activation Test with Different Polyethylene Glycols in Patients with Suspected PEG Hypersensitivity Reactions
-
Published:2022-11-23
Issue:23
Volume:23
Page:14592
-
ISSN:1422-0067
-
Container-title:International Journal of Molecular Sciences
-
language:en
-
Short-container-title:IJMS
Author:
Vespa SimoneORCID, Del Biondo Pietro, Simeone PasqualeORCID, Cavallucci Enrico, Catitti Giulia, Auciello Raffaella, De Bellis DomenicoORCID, Altomare Isotta, Pierdomenico Laura, Canonico BarbaraORCID, Cicalini IlariaORCID, Angilletta Ilaria, Del Boccio PieroORCID, Pieragostino DamianaORCID, Santilli FrancescaORCID, Urbani Andrea, De Laurenzi Vincenzo, Stuppia Liborio, Lanuti PaolaORCID
Abstract
Allergic reactions to COVID-19 vaccine components are rare but should be considered. Polyethylene glycol (PEG) is responsible for anaphylaxis in mRNA vaccines. Skin tests have been used in the allergological work-up programs for COVID-19 vaccine evaluation. However, the reproducibility of the skin prick test is time-dependent and the reactivity declines over time. Therefore, we combined the administration of the skin tests with the basophil activation test (BAT) using PEG2000, PEG4000 and DMG-PEG2000, where the BAT was considered positive when the percentage of activated basophils was higher than 6%, 5% and 6.5%, for PEG 4000, PEG2000 and DMG-PEG2000, respectively. To this end, among the subjects that underwent allergy counseling at the Allergy Unit of our Institution during the 2020/2021 vaccination campaign, 13 patients had a suggested medical history of PEG/drug hypersensitivity and were enrolled together with 10 healthy donors. Among the enrolled patients 2 out of 13 tested patients were positive to the skin test. The BAT was negative in terms of the percentages of activated basophils in all analyzed samples, but the stimulation index (SI) was higher than 2.5 in 4 out of 13 patients. These data evidenced that, when the SI is higher than 2.5, even in the absence of positivity to BAT, the BAT to PEG may be a useful tool to be coupled to skin tests to evidence even low-grade reactions.
Funder
Italian Ministry of University and Research (MUR), Italy, FSE-FESR
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
Reference37 articles.
1. Immunology of COVID-19: Mechanisms, Clinical Outcome, Diagnostics, and Perspectives—A Report of the European Academy of Allergy and Clinical Immunology (EAACI);Allergy,2020 2. Maintaining Safety with SARS-CoV-2 Vaccines;N. Engl. J. Med.,2021 3. Immune Response to SARS-CoV-2 and Mechanisms of Immunopathological Changes in COVID-19;Allergy,2020 4. Rossi, C., Lanuti, P., Cicalini, I., De Bellis, D., Pierdomenico, L., Del Boccio, P., Zucchelli, M., Natale, L., Sinjari, B., and Catitti, G. (2021). BNT162b2 MRNA Vaccination Leads to Long-Term Protection from COVID-19 Disease. Vaccines, 9. 5. Lanuti, P., Rossi, C., Cicalini, I., Pierdomenico, L., Damiani, V., Semeraro, D., Verrocchio, S., Del Boccio, P., Evangelista, A., and Sarra, A. (2021). Picture of the Favourable Immune Profile Induced by Anti-SARS-CoV-2 Vaccination. Biomedicines, 9.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|