Abstract
Rare cold inducible 2 (RCI2) proteins are a group of low molecular weight proteins that widely exist in various tissues of plants and play crucial roles in plant growth and development and abiotic stress responses. Genome-wide identification and analysis of RCI2 have not been documented in Rosaceae plants. Therefore, we identified 23 RCI2 genes from seven Rosaceae plants, which were classified into three subfamilies. The RoRCI2 protein encodes a highly conserved domain of Pmp3. Three homologous PmRCI2s genes from Prunus mume were cloned and named PmRCI2-1, PmRCI2-2, and PmRCI2-3. The results of subcellular localization prediction showed that three PmRCI2s localized to membrane structures, and the abscisic acid response element were found to have the largest number in the promoter sequences of PmRCI2s. The results of quantitative real-time PCR (qRT-PCR) showed that PmRCI2-3 was significantly induced by low temperature and highly expressed in stems and buds during the endodormancy stage. Our study improves the understanding of the RCI2 family of Rosaceae plants regarding the cold responses and provides a theoretical basis for the cold-resistant breeding of P. mume.
Funder
the Opening Project of State Key Laboratory of Tree Genetics and Breeding
the National Natural Science Foundation of China
the Special Fund for Beijing Common Construction Project
Subject
Horticulture,Plant Science
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献