Disinfectants Useful to Manage the Emerging Tomato Brown Rugose Fruit Virus in Greenhouse Tomato Production

Abstract

Tomato brown rugose fruit virus (ToBRFV) is an emerging tobamovirus infecting tomato and pepper crops. First identified in 2014 in the Middle East, ToBRFV has spread rapidly around the world. Being seed-borne, resistance breaking and easy mechanical transmission, ToBRFV can spread quickly in a greenhouse through plant handling. Thus, selecting an effective disinfectant that is capable of deactivating virus infectivity is important. We aimed to identify these effective disinfectants for ToBRFV management in greenhouse tomato production, particularly for total cleaning. A useful disinfectant should be effective against ToBRFV infectivity without major phytotoxic effect on the test plants. In this study, we evaluated 11 disinfectants at various concentrations and assessed their efficacy in ToBRFV treatment on tomato plants that were pretreated with or without SP2700, a known antiviral plant activator of Ningnanmycin. SP2700 treated-plants generated systemic acquired resistance with a delay in symptom expression for 2–3 weeks in comparison to the mock control. Overall, 1% Virocid, 2% Virkon S, 0.25% sodium hypochlorite (5% Clorox bleach), and 2.5% trisodium phosphate (TSP) achieved complete deactivation of ToBRFV with 15 min exposure. However, TSP presented serious phytotoxicity. Our results offer practical solutions to manage this emerging disease affecting tomato production in greenhouses.