Affiliation:
1. Division Phytomedicine, Albrecht Daniel Thaer-Institute for Crop and Animal Sciences, Humboldt-Universität zu Berlin, 14195 Berlin, Germany
2. Working Group Biometry and Experimental Methods, Division Crop Science, Albrecht Daniel Thaer-Institute for Crop and Animal Sciences, Humboldt-Universität zu Berlin, 14195 Berlin, Germany
Abstract
The tomato brown rugose fruit virus (ToBRFV) causes severe damage to tomato cultivars and has international economic importance. The harmful tobamovirus is easily mechanically transmissible and highly stable. An ongoing cultivation of infected tomato plants may lead to the spread of ToBRFV in and around the production area of the infested tomato farm. We conducted a study in which we collected a representative number of swab samples from various inanimate surfaces in greenhouses, packaging halls, and shared and private accommodations. In addition, numerous fabrics, such as outer clothing, bed linen, and items used by greenhouse workers, were tested. The infectivity of ToBRFV-contaminated surfaces was tested in bioassays using Nicotiana tabacum cv. Xanthi NN and confirmed using DAS-ELISA. The proportion of ToBRFV-contaminated surfaces varied among locations, from 48.7% in greenhouses to 0% in offices with limited access to staff. Samples from shared accommodation and private accommodation were 18.4% and 3.6% ToBRFV positive, respectively. Clothing and protective items were found to be highly contaminated with ToBRFV, and even around the sleeping area, infective ToBRFV was detected in a few apartments. This study provides evidence for the first time on how and where infectious ToBRFV can be spread by humans beyond the production area. To avoid further dissemination, strict hygiene protocols are required to interrupt transmission routes.
Funder
German Federal Ministry of Food and Agriculture
Deutsche Forschungsgemeinschaft
Open Access Publication Fund of Humboldt-Universität zu Berlin
Subject
Horticulture,Plant Science
Reference61 articles.
1. Tomato brown rugose fruit virus: An emerging and rapidly spreading plant RNA virus that threatens tomato production worldwide;Zhang;Mol. Plant Pathol.,2022
2. First report of tomato brown rugose fruit virus infecting tomato in Germany;Menzel;New Dis. Rep.,2019
3. (2023, February 20). Tomato Brown Rugose Fruit Virus (ToBRFV). Auftretensmeldungen. Available online: https://pflanzengesundheit.julius-kuehn.de/tomato-brown-rugose-fruit-virus.html.
4. First report of Pepino mosaic virus on tomato;Stijger;Plant Dis.,2000
5. Torradoviruses;Verbeek;Annu. Rev. Phytopathol.,2015