Genome-Wide Identification and Expression Analysis of HSP70 Gene Family in Chrysanthemum lavandulifolium under Heat Stress

Abstract

As a molecular chaperone, HSP70 is widely involved in complex activities in plants. Under high temperature, drought, high salt, low temperature, heavy metals, and other stresses, HSP70 is rapidly synthesizes, stabilizes protein and biological macromolecular structures, and improves the stress resistance of plants. In this study, 83 ClHSP70 genes in Chrysanthemum lavandulifolium were identified based on the published Chrysanthemum lavandulifolium genome database. The genes were divided into six clusters based on a phylogenetic analysis, and the gene structures, conserved motifs, and functional domains were relatively conserved. Only two collinear genes were observed, and they formed a pair of duplicating genes. Multiple abiotic stress and phytohormone response elements were observed on the ClHSP70 promoter, such as temperature, drought, methyl jasmonate, abscisic acid, and other stress-related elements, and CpG islands were found on some ClHSP70 promoters, suggesting that they may be related to methylation modifications. Tissue expression analysis showed that the ClHSP70 genes were highly expressed in the roots. In addition, the gene expression changed significantly after 1 h of heat treatment and plays an important role in plant responses to temperature stress. The results of this study provide insights on the ClHSP70 gene family members and a theoretical basis for further research on functional analyses of the ClHSP70 gene family.