Affiliation:
1. Faculty of Technology, University of Niš, BulevarOslobođenja 124, 16000 Leskovac, Serbia
2. Faculty of Agriculture, University of Priština in Kosovska Mitrovica, Kopaonička bb, 38219 Lešak, Serbia
3. Institute of Food Technology, University of Novi Sad, 21000 Novi Sad, Serbia
Abstract
The purpose of this study was to determine essential oil yield, composition, and antioxidant activity during two different maturation stages of umbels with seeds(I stage: premature–waxy; and II stage: mature–fully ripening) of wild carrot (Daucus carrota var. carota) from the Montenegrin coast. A higher yield of carrot essential oil (CEO) was determined in mature, fully ripening umbels (1.96 mL/100 g p.m) than in premature umbels at the waxy stage (mL/100 g p.m). Thirty-three components were identified in premature umbels, with β-bisabolene (32.3%), 11-α-(H)-himachal-4-en-1-β-ol (27.9%), elemicin (10.1%), and α-longipipene (7.7%) being the main components. They were followed by α-pinene (3.7%), (E)-asarone (3.4%), (E)-anethole (3.2%), and β-himachalene (2.0%). Thirty-two components were identified in CEO from mature umbels, with β-bisabolene (41.0%), 11-α-(H)-himachal-4-en-1-β-ol(21.1%), elemicin (14.8%), andα-longipipene (5.7%) being the most abundant. These components were followed by (E)-asarone (3.9%), cis-α-bisabolene (2.4%), and β-himachalene (2.0%). The CEO isolated from mature umbelsshowed better antioxidant activity (EC50 value of 31.80 mg/mL) in comparison to the CEO isolated from premature umbels (EC50 value of 49.18 mg/mL) during the incubation time of 60 min. The degree of DPPH radical neutralization increased as the incubation time increased from 20 to 60 min. Therefore, our findings recommend that wild carrot could be harvested in the fully ripening stage when the umbel improves CEO yield and antioxidant activity, without the risk of seed shedding from the umbel and seed losses.
Funder
the Ministry of Education Science and Technological Development of the Republic of Serbia
Subject
Horticulture,Plant Science
Reference43 articles.
1. Pignatti, S. (1982). Flora Ditalia, Edagricole.
2. Van Wyk, B.-E., and Wink, M. (2004). Medicinal Plants of the World, Briza Publications.
3. Hoffman, D. (1990). The New Holistic Herbal, Element Press. [3rd ed.].
4. Essential oils of wild and cultivated carrots-the chemical composition and antimicrobial activity;Staniszewska;J. Essent Oil Res.,2005
5. (E)-methylisoeugenol and elemicin: Antibacterial components of Daucus carota L. essential oils against campylobacter jejuni;Rossi;J. Agric. Food Chem.,2007