Abstract
The use of implant materials is always associated with the risk of infection. Moreover, the effectiveness of antibiotics is reduced due to antibiotic-resistant pathogens. Thus, selecting the appropriate alternative antimicrobials for local delivery systems is correlated with successful infection management. We evaluated immobilization of the S. aureus specific bacteriophages in clinically recognized biopolymers, i.e., chitosan and alginate, to control the release profile of the antimicrobials. The high-titre S. aureus specific bacteriophages were prepared from commercial bacteriophage cocktails. The polymer mixtures with the propagated bacteriophages were then prepared. The stability of the S. aureus bacteriophages in the biopolymer solutions was assessed. In the case of chitosan, no plaques indicating the presence of the lytic bacteriophages were observed. The titre reduction of the S. aureus bacteriophages in the Na-alginate was below 1 log unit. Furthermore, the bacteriophages retained their lytic activity in the alginate after crosslinking with Ca2+ ions. The release of the lytic S. aureus bacteriophages from the Ca-alginate matrices in the TRIS-HCl buffer solution (pH 7.4 ± 0.2) was determined. After 72 h—0.292 ± 0.021% of bacteriophages from the Ca-alginate matrices were released. Thus, sustained release of the lytic S. aureus bacteriophages can be ensured.
Funder
European Regional Development Fund
European Union’s Horizon 2020 research and innovation programme
Subject
Molecular Biology,Biochemistry
Reference45 articles.
1. Lu, Y., Cai, W.J., Ren, Z., and Han, P. (2022). The Role of Staphylococcal Biofilm on the Surface of Implants in Orthopedic Infection. Microorganisms, 10.
2. Season of the year influences infection rates following total hip arthroplasty;Rosas;World J. Orthop.,2017
3. Scaffold-based anti-infection strategies in bone repair;Johnson;Ann. Biomed. Eng.,2015
4. Oral antibiotic treatment of staphylococcal bone and joint infections in adults;Kim;J. Antimicrob. Chemother.,2014
5. Recent advances in the local antibiotics delivery systems for management of osteomyelitis;Wassif;Drug Deliv.,2021
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献