Microbial Poly(hydroxybutyrate-co-hydroxyvalerate) Scaffold for Periodontal Tissue Engineering

Author:

Phuegyod Seubsakul1ORCID,Pramual Sasivimon2ORCID,Wattanavichean Nungnit3ORCID,Assawajaruwan Supasuda1,Amornsakchai Taweechai4,Sukho Panithi5,Svasti Jisnuson2ORCID,Surarit Rudee67,Niamsiri Nuttawee1

Affiliation:

1. Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

2. Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok 10210, Thailand

3. School of Materials Science and Innovation, Faculty of Science, Mahidol University, Nakhon Pathom 73170, Thailand

4. Center of Excellence for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Mahidol University, Nakhon Pathom 73170, Thailand

5. Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand

6. Department of Oral Biology, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand

7. Faculty of Dentistry, Siam University, Bangkok 10160, Thailand

Abstract

In this study, we fabricated three dimensional (3D) porous scaffolds of poly(hydroxybutyrate-co-hydroxyvalerate) with 50% HV content. P(HB-50HV) was biosynthesized from bacteria Cupriavidus necator H16 and the in vitro proliferation of dental cells for tissue engineering application was evaluated. Comparisons were made with scaffolds prepared by poly(hydroxybutyrate) (PHB), poly(hydroxybutyrate-co-12%hydroxyvalerate) (P(HB-12HV)), and polycaprolactone (PCL). The water contact angle results indicated a hydrophobic character for all polymeric films. All fabricated scaffolds exhibited a high porosity of 90% with a sponge-like appearance. The P(HB-50HV) scaffolds were distinctively different in compressive modulus and was the material with the lowest stiffness among all scaffolds tested between the dry and wet conditions. The human gingival fibroblasts (HGFs) and periodontal ligament stem cells (PDLSCs) cultured onto the P(HB-50HV) scaffold adhered to the scaffold and exhibited the highest proliferation with a healthy morphology, demonstrating excellent cell compatibility with P(HB-50HV) scaffolds. These results indicate that the P(HB-50HV) scaffold could be applied as a biomaterial for periodontal tissue engineering and stem cell applications.

Funder

National Science and Technology Development Agency (NSTDA) through Coordinating Center for Thai Government Science and Technology Scholarship Students

CSTS-MOST, Thailand Science Research and Innovation (TSRI), Chulabhorn Research Institute

Faculty of Science, Mahidol University

Publisher

MDPI AG

Subject

Polymers and Plastics,General Chemistry

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