Affiliation:
1. Department of Engineering, China University of Petroleum-Beijing at Karamay, Karamay 834000, China
2. State Key Laboratory of Heavy Oil Processing-Karamay Branch, Karamay 834000, China
3. Department of Petroleum, China University of Petroleum-Beijing at Karamay, Karamay 834000, China
Abstract
Quercetin, as one of the most biologically active natural flavonoids, is widely found in various vegetables, fruits and Chinese herbs. In this work, molecularly imprinted polymer (MIP) was synthesized through surface molecular imprinting technology with sol-gel polymerization mechanism on SiO2 at room temperature using quercetin as the template, SiO2 as the supporter, 3-aminopropyltriethoxysilane (APTES) as the functional monomer, and tetraethoxysilane (TEOS) as the cross-linker. The prepared MIP was characterized via scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FT-IR) and nitrogen adsorption measurements to validate its surface morphology, structure and functionality. SEM images revealed that the morphology of MIP was rough and spherical with the particle size of 260 nm larger than that of the support SiO2. In the FTIR spectra of MIP, the band around 1499 cm−1 and 2932 cm−1 were assigned to N−H and C-H groups, respectively. The results indicated that the imprinted polymer layers were grafted on the surface of SiO2 and the MIP had been successfully prepared. Since the specific surface area and pore volume of MIP were markedly higher than those of NIP and SiO2 and were 52.10 m2 g−1 and 0.150 cm3 g−1, respectively, it was evident that the imprinting process created corresponding imprinted cavities and porosity. The MIP for adsorbing quercetin was evaluated by static adsorption experiment. The results indicated that the adsorption equilibrium could be reached within 90 min and the maximum adsorption capacity was as high as 35.70 mg/g. The mechanism for adsorption kinetics and isotherm of MIP for quercetin was proved to conform the pseudo-second-order kinetics model (R2 = 0.9930) and the Freundlich isotherm model (R2 = 0.9999), respectively, revealing that chemical adsorption and heterogeneous surface with multilayer adsorption dominated. In contrast to non-imprinted polymer (NIP), the MIP demonstrated high selectivity and specific recognition towards quercetin whose selectivity coefficients for quercetin relative to biochanin A were 1.61. Furthermore, the adsorption capacity of MIP can be maintaining above 90% after five regeneration cycles, indicating brilliant reusability and potential application for selective adsorption of quercetin.
Funder
Xinjiang Uygur Autonomous Region Science and Technology Program
Subject
Polymers and Plastics,General Chemistry
Reference50 articles.
1. Effects of dietary quercetin on growth, antioxidant capacity, immune response and immune-related gene expression in snakehead fish, Channa argus;Kong;Aquac. Rep.,2022
2. Quercetin attenuates cisplatin-induced ovarian toxicity in rats: Emphasis on anti-oxidant, anti-inflammatory and anti-apoptotic activities;Algandaby;Arab. J. Chem.,2021
3. Anti-tumor potential of nitroxyl derivative Pirolin in the DMBA-induced rat mammary carcinoma model: A comparison with quercetin;Tabaczar;Pharmacol. Rep.,2015
4. Preparation, characterization and usage of molecularly imprinted polymer for the isolation of quercetin from hydrolyzed nettle extract;Apak;J. Chromatogr. B,2016
5. A selective artificial enzyme inhibitor based on nanoparticle-enzyme interactions and molecular imprinting;Zheng;Adv. Mater.,2013
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献