Organo-Mineral Interactions Are More Important for Organic Matter Retention in Subsoil Than Topsoil

Abstract

Decomposing crop residues contribute to soil organic matter (SOM) accrual; however, the factors driving the fate of carbon (C) and nitrogen (N) in soil fractions are still largely unknown, especially the influence of soil mineralogy and autochthonous organic matter concentration. The objectives of this work were (1) to evaluate the retention of C and N from crop residue in the form of occluded and mineral-associated SOM in topsoil (0–20 cm) and subsoil (30–70 cm) previously incubated for 51 days with 13C-15N-labelled corn residues, and (2) to explore if specific minerals preferentially control the retention of residue-derived C and N in topsoil and subsoil. We used topsoil and subsoil having similar texture and mineralogy as proxies for soils being rich (i.e., topsoil) and poor (i.e., subsoil) in autochthonous organic matter. We performed a sequential density fractionation procedure and measured residue-derived C and N in occluded and mineral-associated SOM fractions, and used X-ray diffraction analysis of soil density fractions to investigate their mineralogy. In accordance with our hypothesis, the retention of C and N from crop residue through organo-mineral interactions was greater in subsoil than topsoil. The same minerals were involved in the retention of residue-derived organic matter in topsoil and subsoil, but the residue-derived organic matter was associated with a denser fraction in the subsoil (i.e., 2.5–2.6 g cm−3) than in the topsoil (i.e., 2.3–2.5 g cm−3). In soils and soil horizons with high clay content and reactive minerals, we find that a low SOM concentration leads to the rapid stabilization of C and N from newly added crop residues.