Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of Croceibacterium aestuarii sp. nov., a Novel Carotenoid-Producing Species in the Family Erythrobacteraceae
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Published:2024-01-03
Issue:1
Volume:12
Page:99
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ISSN:2077-1312
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Container-title:Journal of Marine Science and Engineering
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language:en
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Short-container-title:JMSE
Author:
Sun Xiao-Yan12, Dong Han12, Zhang Yu12, Gao Jia-Wei12, Zhou Peng3, Sun Cong12, Xu Lin12ORCID
Affiliation:
1. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China 2. Shaoxing Biomedical Research Institute of Zhejiang Sci-Tech University Co., Ltd., Zhejiang Engineering Research Center for the Development Technology of Medicinal and Edible Homologous Health Food, Shaoxing 312075, China 3. Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, China
Abstract
Carotenoids are extensively used in drugs, cosmetics, nutrients, and foods, owing to their antioxidant and anti-inflammatory characteristics. Diverse marine heterotrophic prokaryotes can accumulate carotenoids and become promising alternatives for the advancement of carotenoids production. In this research, 55 strains were isolated and cultivated from tidal flat sediment in Zhoushan and classified into the phyla Pseudomonadota (n = 24), Bacillota (n = 18), Bacteroidota (n = 9), and Actinomycetota (n = 4). Nine of them accumulated carotenoids, and most of them belonged to the families Flavobacteriaceae (n = 4) and Erythrobacteraceae (n = 4). Among those carotenoid-producing strains, one strain, designated as D39T, was proposed as one novel species belonging to the genus Croceibacterium through polyphasic taxonomy approaches. Genomic annotations and carotenoid compound determinations revealed that strain D39T encoded crtEBIYZG genes and mainly accumulated zeaxanthin as major carotenoids. Furthermore, carotenoid biosynthesis pathway in the majority of Croceibacterium strains were identical with that in the strain D39T, implying that Croceibacterium members can be sources of producing zeaxanthin. This study enhances knowledge of microbial biodiversity in tidal flats, proposes a novel carotenoid-producing Croceibacterium species, and elucidates carotenoid biosynthesis pathway in the genus Croceibacterium, which contribute to enriching marine carotenoid-producing strains and promoting a comprehensive insight into genomic contents of them.
Funder
National Natural Science Foundation of China Zhejiang Provincial Natural Science Foundation of China National Science and Technology Fundamental Resources Investigation Program of China Fundamental Research Funds of Zhejiang Sci-Tech University Open Fund of Zhejiang Sci-Tech University Shaoxing Academy of Biomedicine Co., Ltd.
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