Structure Characterization and Functional Properties of Flaxseed Protein–Chlorogenic Acid Complex
Author:
Cao Weiwei1ORCID,
Chen Junliang1,
Ma Shuhua1,
Chen Xin1,
Dai Xin1,
Zhang Li1,
Guo Mengyao1,
Li Linlin1,
Liu Wenchao1,
Ren Guangyue1,
Duan Xu1ORCID,
Xie Qinggang2
Affiliation:
1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
2. Heilongjiang Feihe Dairy Co., Ltd., Beijing 100015, China
Abstract
This study aimed to investigate the effects of the covalent binding of flaxseed protein (FP) and chlorogenic acid (CA) on the structure and functional properties of FP–CA complexes fabricated using the alkali method. The results suggested that the encapsulation efficiency of CA encapsulated by FP ranged from 66.11% to 72.20% and the loading capacity of CA increased with an increasing addition ratio of CA with a dose-dependent relationship, which increased from 2.34% to 10.19%. The particle size, turbidity, zeta potential and PDI of FP and the FP–CA complexes had no significant discrepancy. UV–Vis and fluorescence spectra showed the existence of the interaction between FP and CA. SEM images showed that the surface of the FP–0.35%CA complex had more wrinkles compared to FP. Differential scanning calorimetry analysis indicated the decomposition temperature of FP at 198 °C was higher than that (197 °C) of the FP–0.35%CA complex, implying that the stability of the FP–CA complexes was lower than FP. The functional properties suggested that the FP–CA complex with 1.40% CA had a higher water holding capacity (500.81%), lower oil holding capacity (273.495%) and lower surface hydrophobicity. Moreover, the FP–CA complexes had better antioxidant activities than that of FP. Therefore, this study provides more insights for the potential application of FP–CA covalent complexes in functional food processing.
Funder
Natural Science Foundation of Henan
Young Key Teachers in University in Henan Province
Key Science and Technology Program of Henan Province
Special Program for the Introduction of Foreign Intelligence in Henan Province
Henan University of Science and Technology
Subject
Plant Science,Health Professions (miscellaneous),Health (social science),Microbiology,Food Science