Isoespintanol Antifungal Activity Involves Mitochondrial Dysfunction, Inhibition of Biofilm Formation, and Damage to Cell Wall Integrity in Candida tropicalis
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Published:2023-06-15
Issue:12
Volume:24
Page:10187
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ISSN:1422-0067
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Container-title:International Journal of Molecular Sciences
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language:en
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Short-container-title:IJMS
Author:
Contreras Martínez Orfa Inés1ORCID, Angulo Ortíz Alberto2, Santafé Patiño Gilmar2ORCID, Peñata-Taborda Ana3ORCID, Berrio Soto Ricardo1
Affiliation:
1. Biology Department, Faculty of Basic Sciences, Universidad de Córdoba, Montería 230002, Colombia 2. Chemistry Department, Faculty of Basic Sciences, Universidad de Córdoba, Montería 230002, Colombia 3. Biomedical and Molecular Biology Research Group, Universidad del Sinú E.B.Z., Montería 230001, Colombia
Abstract
The growing increase in infections caused by C. tropicalis, associated with its drug resistance and consequent high mortality, especially in immunosuppressed people, today generates a serious global public health problem. In the search for new potential drug candidates that can be used as treatments or adjuvants in the control of infections by these pathogenic yeasts, the objective of this research was to evaluate the action of isoespintanol (ISO) against the formation of fungal biofilms, the mitochondrial membrane potential (ΔΨm), and its effect on the integrity of the cell wall. We report the ability of ISO to inhibit the formation of biofilms by up to 89.35%, in all cases higher than the values expressed by amphotericin B (AFB). Flow cytometric experiments using rhodamine 123 (Rh123) showed the ability of ISO to cause mitochondrial dysfunction in these cells. Likewise, experiments using calcofluor white (CFW) and analyzed by flow cytometry showed the ability of ISO to affect the integrity of the cell wall by stimulating chitin synthesis; these changes in the integrity of the wall were also observed through transmission electron microscopy (TEM). These mechanisms are involved in the antifungal action of this monoterpene.
Funder
FCB-02-19 project of the University of Córdoba, Montería, Colombia
Subject
Inorganic Chemistry,Organic Chemistry,Physical and Theoretical Chemistry,Computer Science Applications,Spectroscopy,Molecular Biology,General Medicine,Catalysis
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