1. Postgraduate Program in Microbiology, Parasitology and Pathology, Biological Sciences, Department of Basic Pathology, Federal University of Parana, Curitiba 81531-980, Brazil
2. Postgraduate Program in Bioprocess and Biotechnology Engineering, Technology Sector, Department of Biotechnology, Federal University of Parana, Curitiba 81531-990, Brazil
3. Regional Epidemiological Laboratory, Faculty of Health Sciences, National University of the East, Minga Guazú 7420, Paraguay
4. Ministry of Public Health and Social Welfare, Asuncion 2160, Paraguay
5. Department of Public Health, Federal University of Latin American Integration, Foz do Iguaçu 85870-650, Brazil
6. Department of Public Health, Hospital de Clínicas, Federal University of Paraná, Curitiba 80060-900, Brazil