Enrichment of Denitrifying Bacterial Community Using Nitrite as an Electron Acceptor for Nitrogen Removal from Wastewater

Abstract

This work aimed to enrich a denitrifying bacterial community for economical denitrification via nitrite to provide the basic objects for enhancing nitrogen removal from wastewater. A sequencing batch reactor (SBR) with continuous nitrite and acetate feeding was operated by reasonably adjusting the supply rate based on the reaction rate, and at a temperature of 20 ± 2 °C, pH of 7.5 ± 0.2, and dissolved oxygen (DO) of 0 mg/L. The results revealed that the expected nitrite concentration can be achieved during the whole anoxic reaction period. The nitrite denitrification rate of nitrogen removal from synthetic wastewater gradually increased from approximately 10 mg/(L h) to 275.35 mg/(L h) over 12 days (the specific rate increased from 3.83 mg/(g h) to 51.80 mg/(g h)). Correspondingly, the chemical oxygen demand/nitrogen (COD/N) ratio of reaction decreased from 7.9 to 2.7. Both nitrite and nitrate can be used as electron acceptors for denitrification. The mechanism of this operational mode was determined via material balance analysis of substrates in a typical cycle. High-throughput sequencing showed that the main bacterial community was related to denitrification, which accounted for 84.26% in the cultivated sludge, and was significantly higher than the 2.16% in the seed sludge.