Affiliation:
1. Department Microbiology and Environmental Biotechnologies, Faculty of Plant Protection and Agroecology, Agricultural University—Plovdiv, 12 Mendeleev Str., 4000 Plovdiv, Bulgaria
Abstract
The accumulation of sewage sludge is a severe problem in many countries. Its utilization through composting has the potential to become a widely applied technology. From this perspective, our study investigated the diversity of fungi in mesophilic and thermophilic habitats when composting biosolids, cow manure and wheat straw. It was conducted using a metagenomic approach and next-generation Illumina HiSeq2000 sequencing to reveal the fungal diversity. We found significantly enhanced microbial activity in the thermophilic phase. In contrast, the activity of enzyme β-glucosidase was 29% higher in the mesophilic zone. The range of α-diversity values was more pronounced in the mesophilic habitats than in the thermophilic habitats based on diversity indices. At the class level, the mesophilic fungi were represented by Sordariomycetes—58.7%, Pezizomycetes—15.1% and Agaricomycetes—12.3%, while the most abundant thermophilic fungi found were Sordariomycetes—39.5% and Pezizomycetes—9.8%. In the further clarification of genera diversity, it is striking that at 37.2 °C, Psathyrella was the most abundant with 35.91%, followed by Chaetomidium with 20.11%. Among the thermophiles, Thielavia and Mortierella were the most common. Further research on microbial diversity changes over time is needed to manage the metabolic processes in obtaining quality soil amendment.
Funder
Bulgarian Ministry of Education and Science
Subject
Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science
Cited by
2 articles.
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