Seasonal Variations in Semen Quality, Testosterone Levels, and Scrotal Size following Dietary Flaxseed Oil and Ascorbic Acid in South African Indigenous Rams
Author:
Ngcobo Jabulani Nkululeko12ORCID, Nedambale Tshimangadzo Lucky12, Mpofu Takalani Judas1ORCID, Nephawe Khathutshelo Agree1ORCID, Chokoe Tlou Caswell3, Ramukhithi Fhulufhelo Vincent12ORCID
Affiliation:
1. Department of Animal Sciences, Tshwane University of Technology, Private Bag X680, Pretoria 0001, South Africa 2. Agricultural Research Council, Germplasm, Conservation, Reproductive Biotechnologies, Private Bag X02, Irene 0062, South Africa 3. Department of Agriculture, Land Reform and Rural Development, Directorate Farm Animal Genetic Resource, Private Bag X250, Pretoria 0001, South Africa
Abstract
The purpose of this study was to determine the seasonal variations in semen quality, testosterone levels, and scrotal size, following dietary flaxseed oil and ascorbic acid in South African indigenous rams. A total of 22 South African indigenous rams were randomly distributed into five treatment diets from June 2021 to May 2022 (12 months). To allow for the spermatogenesis period, semen was collected after sixty days of dietary supplementation with treatment diets. Blood was collected twice a week using an 18-gauge needle and vacutainer tubes and sent to the laboratory for testosterone analysis. Semen and blood collection were repeated eight times each season. The scrotal size (circumference, length, and width) was measured using a flexible measuring tape. Data was subjected to the General Linear Model (GLM) in Minitab® 2017. Treatment means were separated using Fisher’s t-test and considered significantly different when the p-value was less than 0.05. Seasons and diet had an effect on progression, total motility, and testosterone levels. For instance, NC during the spring season had the lowest progressive motility (42.84 ± 5.32), followed by the summer (49.38 ± 4.49), winter (62.46 ± 4.35), and autumn (63.26 ± 3.58). Notably, when treatment diets were introduced, improvements were realized, and there were significant differences (p < 0.05) among the seasons following supplementation of FLAX, ASCA, and FLAX + ASCA, except for FLAX in the autumn season (53.83 ± 4.16). Total motility did not differ significantly (p > 0.05) between the seasons when the NC and PC diets were supplemented; nevertheless, there was an improvement when FLAX, ASCA, and FLAX + ASCA were supplemented. Testosterone levels were significantly influenced by the seasons when negative and PC diets were supplemented. It is noteworthy that supplementing FLAX + ASCA can reverse the influence of the season on the testosterone levels (spring, 27.52 ± 4.42; summer, 20.23 ± 5.11; autumn, 25.24 ± 3.96; and winter, 25.92 ± 4.42). In conclusion, seasons do affect semen quality and testosterone levels of South African indigenous rams. However, flaxseed oil and ascorbic acid can reverse the seasonal variations in semen quality and testosterone levels.
Funder
Department of Agriculture, Land Reform and Rural Development
Subject
General Veterinary,Animal Science and Zoology
Reference76 articles.
1. FAO (2022). The State of Food Security and Nutrition in the World 2022, FAO. 2. Ndlovu, M., Clulow, A.D., Savage, M.J., Nhamo, L., Magidi, J., and Mabhaudhi, T. (2021). An Assessment of the Impacts of Climate Variability and Change in KwaZulu-Natal Province, South Africa. Atmosphere, 12. 3. Morphological Structure of Zulu Sheep Based on Principal Component Analysis of Body Measurements;Mavule;Small Rumin. Res.,2013 4. Ngcobo, J.N., Nedambale, T.L., Nephawe, K.A., Mpofu, T.J., Chokoe, T.C., and Ramukhithi, F.V. (2022). An Update on South African Indigenous Sheep Breeds’ Extinction Status and Difficulties during Conservation Attempts: A Review. Diversity, 14. 5. Mezzera, M. Domestic Animal Diversity Information System (DAD-IS)—User Manual, Food and Agriculture Organization of the United Nations.
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