Study on Morphological Changes and Interference in the Development of Aedes aegypti Caused by Some Essential Oil Constituents

Author:

Serdeiro Michele Teixeira12,Dias Thiago Dutra23,de Lima Natanael Teles Ramos4,Barbosa-Filho José Maria4ORCID,Belato Renato de Souza15ORCID,Santos-Mallet Jacenir Reis dos15ORCID,Maleck Marise2367

Affiliation:

1. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil

2. Laboratório de Insetos Vetores, Campus Vassouras and Campus Maricá, Universidade de Vassouras, Vassouras 27700-000, Brazil

3. Mestrado Profissional em Ciências Ambientais, Universidade de Vassouras, Rio de Janeiro 27700-000, Brazil

4. Laboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, João Pessoa 58000-900, Brazil

5. Laboratório de Vigilância e Biodiversidade em Saúde, Universidade de Iguaçu, Nova Iguaçu 26260-045, Brazil

6. Laboratório de Entomologia Médica e Forense, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21040-900, Brazil

7. Colégio Pedro II, Campo de São Cristóvão, Rio de Janeiro 20921-440, Brazil

Abstract

Dengue, Chikungunya and Zika are arboviruses, transmitted by the mosquito Aedes aegypti, that cause high mortality and serious health consequences in human populations. Efforts to control Ae. aegypti are important for preventing outbreaks of these diseases. Essential oil constituents are known to exhibit many activities, such as their use as larvicides. Given their potential, the present study aimed to characterize the larvicidal effect of dihydrojasmone, p-cymene, carvacrol, thymol, farnesol and nerolidol on the larvae of Ae. aegypti and their interference over the morphology of the mosquitos. The essential oil constituents were dissolved in dimethylsulfoxide at concentrations of 1–100 μg/mL and were applied in the breeding environment of third-stage larvae. The larvae from bioassays were fixed, dehydrated and embedded. Ultrathin sections were contrasted using 5% uranyl acetate and 1% lead citrate for observation through transmission electron microscopy. The oil with the highest larvicidal efficiency was found to be nerolidol, followed by farnesol, p-cymene, carvacrol, thymol and dihydrojasmone, with an LC50 of 11, 21, 23, 40, 45 and 66 µg/mL, respectively. The treated Ae. aegypti larvae caused alteration to the tegument or internal portions of larvae. The present study demonstrated which of these oils—dihydrojasmone, farnesol, thymol, p-cymene, carvacrol and nerolidol—have effective larvicidal activity.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil

Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro

Publisher

MDPI AG

Subject

Infectious Diseases,Public Health, Environmental and Occupational Health,General Immunology and Microbiology

Reference48 articles.

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3. MS Ministério da Saúde (2023, May 28). Febre de Chikungunya: Manejo Clínico, Available online: http://bvsms.saude.gov.br/bvs/publicacoes/febre_chikungunya_manejo_clinico.pdf.

4. PAHO Pan American Health Organization (2023, May 28). Epidemiological Alert: Neurological Syndrome, Congenital Malformations, and Zika Virus Infection. Available online: https://iris.paho.org/handle/10665.2/50697.

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