Morphological analysis and lipid composition of the stable fly Stomoxys calcitrans salivary glands reinforce important features of feeding behaviour

Author:

Florencio Melissa12ORCID,Oilveira Magalhães Francisco Romulo12ORCID,Araujo Zuma Aline3ORCID,Lima Oliveira Lugathe Cinara4,Rosa Dayana12ORCID,Riguete Karina12,Machado Motta Maria Cristina35ORCID,Alves do Nascimento Aparecida6ORCID,Reis dos Santos Mallet Jacenir789ORCID,Correa Atella Georgia4ORCID,Fampa Patrícia1ORCID

Affiliation:

1. Departamento de Ciências Farmacêuticas, Instituto de Ciências Biológicas e da Saúde Universidade Federal Rural do Rio de Janeiro Seropédica Brazil

2. Programa de Pós graduação em Ciências Veterinárias, Instituto de Veterinária Universidade Federal Rural do Rio de Janeiro Seropédica Brazil

3. Laboratório de Ultraestrutura Celular Hertha Meyer, Centro de Pesquisa em Medicina de Precisão (CPMP), Instituto de Biofísica Carlos Chagas Filho Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil

4. Instituto de Bioquímica Médica Leopoldo de Meis Universidade Federal do Rio de Janeiro Rio de Janeiro Brazil

5. Centro Nacional de Biologia Estrutural e Bioimagem – CENABIO ‐ RJ Brazil

6. Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde Universidade Federal Rural do Rio de Janeiro Seropédica Brazil

7. Instituto Oswaldo Cruz Fundação Oswaldo Cruz Rio de Janeiro Brazil

8. Fiocruz‐Piauí Teresina Brazil

9. Universidade Iguaçu—UNIG Nova Iguaçu Brazil

Abstract

AbstractStomoxys calcitrans L. (Diptera: Muscidae), the stable fly, is a hematophagous insect of great veterinary importance, because it is a mechanical vector of diverse pathogens in livestock. The saliva of blood‐feeding insects presents important pharmacologically active molecules that impair blood clotting, promote vasodilation and modulate the host immune system response, crucial processes for successful feeding. These properties also enable pathogens' transmission. In the present work, we describe an efficient protocol to dissect S. calcitrans salivary glands, their morphological characteristics and lipid profile. The mean length of the tubular gland is 3.23 mm with a bulbous posterior end and a narrow anterior end. Histological analysis revealed a monolayer of large polygonal epithelial cells with voluminous nuclei and high lipid content in their cytoplasm. Ultrastructural analysis showed that the epithelium is rich in mitochondria, free ribosomes, Golgi complex cisternae, presenting a great extension of rough endoplasmic reticulum that contains an electron‐dense material. Lipid analysis by thin‐layer chromatography showed that neutral fatty acids and phosphatidylcholine are predominant in the fly salivary glands. Lysophosphatidylcholine, an important signalling biomolecule involved in different metabolic processes, including host's immunomodulation and pathogens proliferation and differentiation, is also present.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

Wiley

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