Construction of a Hantaan Virus Phage Antibody Library and Screening for Potential Neutralizing Activity
Author:
Li Zhuo12, Zhang Huiyuan13ORCID, Yu Xiaxia13, Zhang Yusi1ORCID, Chen Lihua1
Affiliation:
1. Department of Immunology, The Fourth Military Medical University, 169 Changle West Road, Xi’an 710032, China 2. Department of Medical Laboratory Technology, Xi’an Health School, Xi’an 710054, China 3. Department of Immunology, Medicine School, Yan’an University, Yan’an 716000, China
Abstract
China is one of the main epidemic areas for hemorrhagic fever with renal syndrome (HFRS). Currently, there is no human antibody specific to Hantaan virus (HTNV) for the emergency prevention and treatment of HFRS. To prepare human antibodies with neutralizing activity, we established an anti-HTNV phage antibody library using phage display technology by transforming peripheral blood mononuclear cells (PBMCs) of patients with HFRS into B lymphoblastoid cell lines (BLCLs) and extracting cDNA from BLCLs that secreted neutralizing antibodies. Based on the phage antibody library, we screened HTNV-specific Fab antibodies with neutralizing activities. Our study provides a potential way forward for the emergency prevention of HTNV and specific treatment of HFRS.
Funder
National Natural Science Foundation of China Natural Science Basic Research Plan in Shaanxi Province of China
Subject
Virology,Infectious Diseases
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