Proteome Analysis Related to Unsaturated Fatty Acid Synthesis by Interfering with Bovine Adipocyte ACSL1 Gene

Author:

Bai Yanbin1,Li Jingsheng1,Wei Yali1,Chen Zongchang1,Liu Zhanxin1,Guo Dashan1,Jia Xue1,Niu Yanmei1,Shi Bingang1,Zhang Xiaolan1,Zhao Zhidong1ORCID,Hu Jiang1,Han Xiangmin1,Wang Jiqing1ORCID,Liu Xiu1,Li Shaobin1ORCID

Affiliation:

1. Gansu Key Laboratory of Herbivorous Animal Biotechnology, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Abstract

Unsaturated fatty acids (UFAs) in beef play a vital role in promoting human health. Long-chain fatty acyl-CoA synthase 1 (ACSL1) is a crucial gene for UFA synthesis in bovine adipocytes. To investigate the protein expression profile during UFA synthesis, we performed a proteomic analysis of bovine adipocytes by RNA interference and non-interference with ACSL1 using label-free techniques. A total of 3558 proteins were identified in both the NC and si-treated groups, of which 1428 were differentially expressed proteins (DEPs; fold change ≥ 1.2 or ≤ 0.83 and p-value < 0.05). The enrichment analysis of the DEPs revealed signaling pathways related to UFA synthesis or metabolism, including cAMP, oxytocin, fatty acid degradation, glycerol metabolism, insulin, and the regulation of lipolysis in adipocytes (p-value < 0.05). Furthermore, based on the enrichment analysis of the DEPs, we screened 50 DEPs that potentially influence the synthesis of UFAs and constructed an interaction network. Moreover, by integrating our previously published transcriptome data, this study established a regulatory network involving differentially expressed long non-coding RNAs (DELs), highlighting 21 DEPs and 13 DELs as key genes involved in UFA synthesis. These findings present potential candidate genes for further investigation into the molecular mechanisms underlying UFA synthesis in bovines, thereby offering insights to enhance the quality of beef and contribute to consumer health in future studies.

Funder

the Young Tutor Support Fund Project of Gansu Agricultural University

the Major Science and Technology Support Project for Cattle and Sheep Industry in Linxia Prefecture

the Discipline Team Project of Gansu Agricultural University

the Efficient Production Technology of Yak in Qilian Mountains Pastoral Areas and Livestock Recycling Development and Demonstration

Publisher

MDPI AG

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