Author:
Tang ZY,Liu ZN,Fu L,Chen DP,Ai QD,Lin Y
Abstract
The aims of our study were to investigate into the effect of
lithium on smooth muscle contraction and phosphorylation of
myosin light chain (MLC20) by MLCK and to find out the clue of its
mechanism. Isolated rabbit duodenum smooth muscle strips
were used to study the effects of lithium on their contractile
activity under the condition of Krebs’ solution by means of HW400S constant temperature smooth muscle trough. Myosin and
MLCK were purified from the chicken gizzard smooth muscle.
Myosin phosphorylation was determined by Glycerol-PAGE,
myosin Mg2+-ATPase activity was measured by Pi liberation
method. Lithium (10-40 mM) inhibited the contraction in
duodenum in a dose-related and time-dependent manner.
Lithium could also inhibit the extent of myosin phosphorylation in
a dose-related and time-dependent manner, whereas it inhibited
Mg2+-ATPase activity in a dose-related manner. Lithium inhibited
smooth muscle contraction by inhibition of myosin
phosphorylation and Mg2+-ATPase activity.
Publisher
Institute of Physiology of the Czech Academy of Sciences
Subject
General Medicine,Physiology
Cited by
8 articles.
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