IN VITRO MORPHOGENETIC REACTION OF MELISSA OFFICINALIS L.-Reference-Cited by-同舟云学术

IN VITRO MORPHOGENETIC REACTION OF MELISSA OFFICINALIS L.

Published:2020-12-12 Issue:1 Volume:1 Page:
ISSN:2734-8083
Container-title:Romanian journal of Horticulture
language:
Short-container-title:RJH

Author:

Radomir Ana Maria¹,Stan Ramona¹

Affiliation:

1. National Research and Development Institute for Biotechnology in Horticulture Stefanesti - Arges

Abstract

Lemon balm (Melissa officinalisL.) is a medicinal plant with a long history in traditional medicine. Classical propagation of this species is inefficient for establishing a good quality clonal plants. The aim of this work was to elaborate an in vitro propagation protocol for M. officinalis using apexes and uninodal fragments as explants. The highest multiplication rate (4.7 shoots/explant) was obtained on a MS medium supplemented with 3 mg/L BAP. A half strength MS medium supplemented with 1 mg/L NAA was the most effective for in vitro rooting of lemon balmmicroshoots. Micropropagated plants transferred ex vitro showed normal morphology and 95% survival rate during acclimatization. The results obtained throughout the in vitro regeneration phases confirm that in vitro tissue culture is an efficient method for multiplication of M. officinalis.

Publisher

Academia de Stiinte Agricole si Silvice 'Gheorghe Ionescu-Sisesti'

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