Affiliation:
1. Vegetable Research and Development Station Bacău
Abstract
The aim of the present research work was the screening of the effect of the main
cytokinin (BAP, kinetin or zeatin) in different concentrations and combinations with
the auxin NAA on androgenesis of white cabbage anthers cultivated in vitro. The
results obtained are regarded as an intermediary stage for the development of a
reproducible protocol for in vitro regeneration of plant from anther culture.
Thus, for the determination of the influence of plant growth regulators formula over
the callus induction and plant regeneration from anthers cultivated in vitro in the
present study the authors undergo a screening of the three most frequently utilized
cytokinins (BAP, kinetin and zeatin) in different concentration and combination with
the auxin NAA. The results obtained, indicated that the best morphogenetic reaction
is obtained on variant with BAP as the main growth regulator.
Publisher
Academia de Stiinte Agricole si Silvice 'Gheorghe Ionescu-Sisesti'