Affiliation:
1. University Ss. Cyril and Methodius, Institute of Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Vodnjanska 17, 1000 Skopje, Republic of Macedonia
2. University Ss. Cyril and Methodius, Institute of Pharmacognosy, Faculty of Pharmacy, Vodnjanska 17, 1000 Skopje, Republic of Macedonia
Abstract
Abstract
An HPLC method for the separation of six target alkaloids from Papaver somniferum L. (morphine, codeine, oripavine, thebaine, papaverine, and noscapine) was developed, optimized, and validated. The chromatographic behavior of these alkaloids was investigated using a reversed-phase chromatography at acidic and alkaline pH. The effects of ion-pairing agents, pH value of the mobile phase, concentration of the buffer components, mobile phase organic modifier, and column temperature were studied. Regardless of the large differences in their pKa values, all alkaloids were separated within a close retention window, and good peak shape was achieved for each of the six alkaloids. The proposed method has adequate selectivity, linearity, accuracy, precision, and reproducibility and is applicable for poppy straw.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry