Isolation, culturing and 3D bioprinting equine myoblasts

Author:

Aimaletdinov AlexanderORCID,Abyzova MariaORCID,Kurilov IgorORCID,Yuferova AlinaORCID,Rutland CatrinORCID,Rizvanov AlbertORCID,Zakirova ElenaORCID

Abstract

Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.

Publisher

Saint Petersburg State University

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Designing cultivated meat: Overcoming challenges in the production process and developing sustainable packaging solutions;Trends in Food Science & Technology;2024-10

2. DEVELOPING READINESS OF TEACHERS TO USE DIGITAL TECHNOLOGIES IN INCLUSIVE EDUCATION;Bulletin of the South Ural State University series "Education. Educational Sciences";2023

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