Affiliation:
1. Department of Chemistry and Pharmacy Pharmaceutical Chemistry Friedrich-Alexander-Universität Erlangen-Nürnberg Nikolaus-Fiebiger-Str. 10 91058 Erlangen Germany
2. Department of Chemistry and Pharmacy, Organic Chemistry II Friedrich-Alexander-Universität Erlangen-Nürnberg Nikolaus-Fiebiger-Str. 10 91058 Erlangen Germany
Abstract
AbstractDespite their long history and their synthetic potential underlined by various recent advances, radical thiol‐yne coupling reactions have so far only rarely been exploited for the functionalization of biomolecules, and no examples yet exist for their application in live cells ‐ although natural thiols show widespread occurrence therein. By taking advantage of the particular cellular conditions of mitochondria in cancer cells, we have demonstrated that radical thiol‐yne coupling represents a powerful reaction principle for the selective targeting of these organelles. Within our studies, fluorescently labeled reactive alkyne probes were investigated, for which the fluorescent moiety was chosen to enable both mitochondria accumulation as well as highly sensitive detection. After preliminary studies under cell‐free conditions, the most promising alkyne‐dye conjugates were evaluated in various cellular experiments comprising analysis by flow cytometry and microscopy. All in all, these results pave the way for improved future therapeutic strategies relying on live‐cell compatibility and selectivity among cellular compartments.
Funder
Friedrich-Alexander-Universität Erlangen-Nürnberg
Deutsche Forschungsgemeinschaft
Subject
General Chemistry,Catalysis,Organic Chemistry
Cited by
2 articles.
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