Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from <i>Streptomyces collinus</i>-Reference-Cited by-同舟云学术

Mechanistic Characterisation of Collinodiene Synthase, a Diterpene Synthase from Streptomyces collinus

Published:2023-10-02 Issue:64 Volume:29 Page:
ISSN:0947-6539
Container-title:Chemistry – A European Journal
language:en
Short-container-title:Chemistry A European J

Author:

Taizoumbe Kizerbo A.¹,Steiner Simon T.¹,Dickschat Jeroen S.¹^ORCID

Affiliation:

1. Kekulé-Institute for Organic Chemistry and Biochemistry University of Bonn Gerhard-Domagk-Straße 1 53121 Bonn Germany

Abstract

AbstractTwo homologs of the diterpene synthase CotB2 from Streptomyces collinus (ScCotB2) and Streptomyces iakyrus (SiCotB2) were investigated for their products by in vitro incubations of the recombinant enzymes with geranylgeranyl pyrophosphate, followed by compound isolation and structure elucidation by NMR. ScCotB2 produced the new compound collinodiene, besides the canonical CotB2 product cyclooctat‐9‐en‐7‐ol, dolabella‐3,7,18‐triene and dolabella‐3,7,12‐triene, while SiCotB2 gave mainly cyclooctat‐9‐en‐7‐ol and only traces of dolabella‐3,7,18‐triene. The cyclisation mechanism towards the ScCotB2 products and their absolute configurations were investigated through isotopic labelling experiments.

Funder

Deutsche Forschungsgemeinschaft

Publisher

Wiley

Subject

General Chemistry,Catalysis,Organic Chemistry

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