Affiliation:
1. Department of Stomatology, the Second Xiangya Hospital Central South University Changsha China
2. National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology The Second Xiangya Hospital of Central South University Changsha China
Abstract
ABSTRACTKangfuxin (KFX) shows potential in wound healing, but its role in socket healing is unclear. This research finds increased bone mass, mineralization, and collagen deposition in KFX‐treated mice. Mouse bone marrow mesenchymal stem cells, human periodontal ligament stem cells (hPDLSCs), and human dental pulp stem cells (hDPSCs) are treated with KFX under osteogenic induction. RNA‐sequencing reveals upregulated chemokine‐related genes, with a threefold increase in chemokine (C‐C motif) ligand 2 (Ccl2). The conditioned medium (CM) of hPDLSCs and hDPSCs treated with KFX promotes endothelial cell migration and angiogenesis. Ccl2 knockdown abolishes CM‐induced endothelial cell migration and angiogenesis, which can be reversed by recombinant CCL2 treatment. KFX‐treated mice showed increased vasculature. In conclusion, KFX increases the expression of CCL2 in stem cells, promoting bone formation and mineralization in the extraction socket by inducing endothelial cell angiogenesis. © 2023 American Society for Bone and Mineral Research (ASBMR).
Funder
Central South University
Hunan Provincial Science and Technology Department
National Natural Science Foundation of China
Publisher
Oxford University Press (OUP)
Subject
Orthopedics and Sports Medicine,Endocrinology, Diabetes and Metabolism
Cited by
1 articles.
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