Abstract
AbstractApproximately 10% of fractures will not heal without intervention. Current treatments can be marginally effective, costly, and some have adverse effects. A safe and manufacturable mimic of anabolic bone is the primary goal of bone engineering, but achieving this is challenging. Mesenchymal stem cells (MSCs), are excellent candidates for engineering bone, but lack reproducibility due to donor source and culture methodology. The need for a bioactive attachment substrate also hinders progress. Herein, we describe a highly osteogenic MSC line generated from induced pluripotent stem cells that generates high yields of an osteogenic cell-matrix (ihOCM) in vitro. In mice, the intrinsic osteogenic activity of ihOCM surpasses bone morphogenic protein 2 (BMP2) driving healing of calvarial defects in 4 weeks by a mechanism mediated in part by collagen VI and XII. We propose that ihOCM may represent an effective replacement for autograft and BMP products used commonly in bone tissue engineering.
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry
Reference70 articles.
1. Morshed, S. Current options for determining fracture union. Adv. Med. 2014, 708574 (2014).
2. Bishop, J. A., Palanca, A. A., Bellino, M. J. & Lowenberg, D. W. Assessment of compromised fracture healing. J. Am. Acad. Orthop. Surg. 20, 273–282 (2012).
3. Rihn, J. A., Kirkpatrick, K. & Albert, T. J. Graft options in posterolateral and posterior interbody lumbar fusion. Spine (Philos. Pa 1976) 35, 1629–1639 (2010).
4. Rosemont, I. L. United States Bone and Joint Decade: The Burden of Musculoskeletal Diseases and Musculoskeletal Injuries (American Academy of Orthopedic Surgeons, 2008).
5. Chrastil, J., Low, J. B., Whang, P. G. & Patel, A. A. Complications associated with the use of the recombinant human bone morphogenetic proteins for posterior interbody fusions of the lumbar spine. Spine (Philos. Pa 1976) 38, E1020–E1027 (2013).
Cited by
41 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献