Production and characterization of active recombinant human factor II with consistent sialylation

Author:

Lee Jeong H.1ORCID,Reier Jason1,Heffner Kelley M.2,Barton Christopher3,Spencer David4,Schmelzer Albert E.1,Venkat Raghavan1

Affiliation:

1. Biopharmaceutical Development; MedImmune; 1 MedImmune Way Gaithersburg 20878 Maryland

2. Department of Chemical and Biomolecular Engineering; Johns Hopkins University; Baltimore Maryland

3. Merrimack Pharmaceuticals; Cambridge Massachusetts

4. Ipsen Biopharm Limited; Wrexham Industrial Estate, Wrexham United Kingdom

Publisher

Wiley

Subject

Applied Microbiology and Biotechnology,Bioengineering,Biotechnology

Reference32 articles.

1. Effects of nutrient levels and average culture pH on the glycosylation pattern of camelid-humanized monoclonal antibody;Aghamohseni;J Biotechnol,2014

2. Decarboxylation of gamma-carboxyglutamic acid residue in human prothrombin. Stoichiometry of calcium binding to gamma-carboxyglutamic acid in prothrombin;Bajaj;J Biol Chem,1982

3. Effects of culture conditions on N-glycolylneuraminic acid (Neu5Gc) content of a recombinant fusion protein produced in CHO cells;Borys;Biotechnol Bioeng,2010

4. Identification of cell culture conditions to control N-glycosylation site-occupancy of recombinant glycoproteins expressed in CHO cells;Gawlitzek;Biotechnol Bioeng,2009

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