Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase‐mediated cassette exchange system-Reference-Cited by-同舟云学术

Development of a targeted integration Chinese hamster ovary host directly targeting either one or two vectors simultaneously to a single locus using the Cre/Lox recombinase‐mediated cassette exchange system

Published:2021-04-09 Issue: Volume: Page:
ISSN:8756-7938
Container-title:Biotechnology Progress
language:en
Short-container-title:Biotechnol Progress

Author:

Ng Domingos¹,Zhou Meixia¹,Zhan Dejin,Yip Shirley¹,Ko Peggy¹,Yim Mandy¹,Modrusan Zora²,Joly John³,Snedecor Brad¹,Laird Michael W.¹,Shen Amy¹^ORCID

Affiliation:

1. Department of Cell Culture and Bioprocess Operations Genentech, Inc. San Francisco California USA

2. DNA Sequencing Lab Genentech, Inc. San Francisco California USA

3. Department of Analytical Development and Quality Control Genentech, Inc. San Francisco California USA

Publisher

Wiley

Subject

Biotechnology

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/btpr.3140

Reference38 articles.

1. Decreasing lactate level and increasing antibody production in Chinese Hamster Ovary cells (CHO) by reducing the expression of lactate dehydrogenase and pyruvate dehydrogenase kinases

2. Optimization of Heavy Chain and Light Chain Signal Peptides for High Level Expression of Therapeutic Antibodies in CHO Cells

3. Enhancing heterologous protein expression and secretion in HEK293 cells by means of combination of CMV promoter and IFNα2 signal peptide

4. Efficient production of bispecific IgG of different isotypes and species of origin in single mammalian cells

5. Generation of monoclonal antibody-producing mammalian cell lines

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