Affiliation:
1. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests College of Plant Protection, Hunan Agricultural University Changsha China
2. National Research Center of Engineering & Technology for Utilization of Botanical Functional Ingredients Hunan Agricultural University Changsha China
3. Hunan Provincial Engineering & Technology Research Center for Biopesticide and Formulation Processing Changsha China
4. Hunan Institute of Plant Protection Changsha China
5. College of Plant Protection South China Agricultural University Guangzhou China
Abstract
AbstractBACKGROUNDThe fall armyworm, Spodoptera frugiperda is one of the most destructive agricultural pests, which can complete their entire life cycle on various plants. At present, some detoxification genes have been proved to be involved in the adaptability to plants in insects. However, the genetics behind insect pest responses to host switches, and their ability to adapt to new host plants, remain poorly understood. This study was conducted to evaluate the adaptation of S. frugiperda to host plant and determine the roles of CYP321A9 and CYP9A58 in the detoxification metabolism of the fall armyworm.RESULTSThe results revealed that feeding on maize was more suitable for S. frugiperda to develop compared with rice. In addition, knocking down of SfCYP321A9 and SfCYP9A58 resulted in a prolonged developmental time of S. frugiperda larvae that fed on rice. Meanwhile, RNAi knockdown of SfCYP321A9 resulted in significantly higher mortality of S. frugiperda larvae when exposed to the rice allelochemicals, ferulic acid, gramine and tricin. Furthermore, overexpression of SfCYP321A9 significantly reduced mortality in Drosophila melanogaster when exposed to gramine and tricin.CONCLUSIONOur results suggest that CYP321A9 and CYP9A58 genes play a key role in host plant adaptation in S. frugiperda, which contribute to a greater understanding of the molecular basis of host plant adaptation and provide the means to develop effective management tools for S. frugiperda resistance. © 2023 Society of Chemical Industry.
Funder
Hunan Agricultural University
Subject
Insect Science,Agronomy and Crop Science,General Medicine
Cited by
5 articles.
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