Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm

Author:

Anthis Nicholas J.1,Clore G. Marius1

Affiliation:

1. Laboratory of Chemical Physics; National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health; Bethesda Maryland 20892-0520

Funder

Intramural Program of the NIH, NIDDK, and the Intramural AIDS Targeted Antiviral Program of the Office of the Director of the NIH (to GMC)

Publisher

Wiley

Subject

Molecular Biology,Biochemistry

Reference20 articles.

1. Quantitation of protein;Stoscheck;Methods Enzymol,1990

2. Spectroscopic determination of tryptophan and tyrosine in proteins;Edelhoch;Biochemistry,1967

3. Calculation of protein extinction coefficients from amino acid sequence data;Gill;Anal Biochem,1989

4. How to measure and predict the molar absorption coefficient of a protein;Pace;Protein Sci,1995

5. Spectrophotometric determination of protein concentration;Grimsley;Curr Protoc Protein Sci Chapter,2004

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